Strain

Genotype

Reference or Source

Comments

WT C4

(Race T)

MAT1-2; Tox1⁺

ATCC 48331

Leach et al. (1982)

Wild type strains, obtained after six backcrosses and are nearly isogenic.

WT C5

(Race O)

MAT1-1; Tox1^-

ATCC 48332

Leach et al. (1982)

cga1

MAT1-1; Tox1^-

ATCC 48332

Horwitz et al. (1999)

G-protein α subunit disrupted mutant, created by insertion of the hygromycin cassette into the coding region, combined with an 18-bp deletion.

C4cga1

MAT1-2; Tox1⁺

ATCC 48331

Horwitz et al. (1999)

G-protein α subunit disrupted mutant, created by complete deletion of the coding region and insertion of hygromycin cassette.

cgb1

MAT1-2; Tox1⁺

ATCC 48331

Ganem et al. (2004)

G-protein β subunit disrupted mutant, created by insertion of the hygromycin cassette into the coding region, combined with a 473 bp deletion. This strain contains an additional mutation but its phenotypic traits are similar in almost every aspect to the cgb1 that contains only one mutation.

cga1 cgb1

MAT1-1; Tox1^-

ATCC 48332

Degani (2013)

G-protein α and β subunits double mutant, created by insertion of the Bar cassette into the CGB1 coding region of cga1 mutant strain, combined with a 473 bp deletion.

chk1

MAT1-2; Tox1⁺

ATCC 48331

Lev et al. (1999)

MAPK disrupted mutant, created by replacement of the coding region with the hygromycin resistance cassette.